Hoi-Ying Holman PhDSenior Staff Scientist LBNLHolman’s group
Berkeley CA 94720
United Statework Work Phone: 510-486-5943
work Work Email: firstname.lastname@example.org
Senior Staff Scientist
Climate and Ecosystem Sciences Division &
Molecular Biophysics and Integrated Bioimaging Division
Director, Synchrotron Infrared Structural Biology (BSISB) Program
Lawrence Berkeley National Laboratory
Key Technologies: synchrotron infrared techniques, Raman microscopy, hyperspectral imaging, microfluidics, chemometrics analysis
Biology Areas: microbial ecology, geomicrobiology, biofuel
Biography: My research focuses on addressing questions central to how microbes shape and are shaped by their immediate environments. I have pioneered a number of experimental methods in synchrotron infrared (SIR) spectromicroscopy of changing chemistry in live microbes at a micrometer spatial resolution and at 10s of seconds temporal resolution. My group also uses other complementary imaging methods including fluorescence, Raman, and VIS/NIR hyperspectral microscopy to achieve a comprehensive study of our biological systems of interest.
As a Director of the BSISB program at the Advanced Light Source (ALS), I collaborate with the SIR technology user-investigators to gain new knowledge of the dissolution and precipitation of minerals by/near microbes, the ecology of biofuel producing microbes, microbe/plant-root-hair interactions, mitochondrial dysfunction, and early chemical and signaling events in migrating mammalian cells. I also coordinate and participate in the development of new Infrared imaging techniques for studying microbial and plant systems.
Current technologies in active development in our BSISB program are SIR nano-spectroscopy, SIR tomography, and SIR plasmon resonance microscopy. A second active area is the development of chemometrics for spectral image analysis; this area is designed to address the challenge of interpreting the descriptive metadata generated from spectral images of biological samples that has become increasingly common with the advent of new SIR spectral microscopies.
A third active technological thrust is microfluidics, which we use to circumvent complications arising from water being a strong mid-infrared absorber. To use IR spectromicroscopy to study live cultured cells, researchers build sophisticated microfluidic devices to overcome this obstacle. In collaboration with BSISB users, we developed a breakthrough technique to easily fabricate SIR-transparent microfluidic devices at a ~10-fold lower production cost. We expect this will have a scientific impact and simplify FTIR imaging of live chemistry in cells.